Living systems are full of rhythms, heartbeats, circadian cycles, neural oscillations, spanning frequencies from many per second to one per day. Lifesong turns those rhythms into music. The artistic mapping from biology to sound is interpretive, but the tuning underneath, how a frequency becomes a pitch and snaps to a musical scale, is exact equal-tempered music theory, and the calibration pins it.
Sonification is the auditory cousin of visualization: representing data as sound so the ear can pick out structure the eye might miss. The choice of how to map a biological frequency to an audible pitch is aesthetic, there is no fact that a circadian rhythm "is" a particular note, but once a mapping is chosen it can be made precise and consistent, which is what makes the result music rather than noise.
Western tuning uses twelve-tone equal temperament. Every pitch corresponds to a MIDI number, with the anchor A4 = 440 Hz = MIDI 69, and the conversion is
midi = 69 + 12 * log2(frequency / 440).
Two facts fall straight out, and the calibration checks both: an octave (doubling the frequency to 880 Hz) raises the MIDI number by exactly twelve semitones, and MIDI 60 converts back to middle C at 261.63 Hz.
A raw frequency rarely lands on a musical note. Lifesong quantizes: it converts the frequency to MIDI, then searches the nearby octaves for the closest pitch belonging to the chosen scale (pentatonic, major, minor, chromatic, or whole-tone) and returns that. So the output is always consonant within the scale. The calibration confirms the snapping: a slightly sharp 263 Hz lands on middle C, and a slightly sharp 450 Hz lands on A4, both in C major.
Frequencies are also clamped to an audible working band, so a rhythm that is too slow or too fast to hear is folded into the hearable range rather than reproduced literally. That is a representational choice, not a distortion of the math.
Each voice is given one of four classic oscillator waveforms, sine, triangle, sawtooth, square. These are mathematical idealizations with simple spectra, not the rich, evolving timbres of real biological sounds. They are the synthesis primitives that make the rhythms audible and distinguishable, nothing more.
Lifesong is an instrument, an aesthetic way to hear the structure of rhythms, not an analysis tool. Its quantitative content is the tuning and quantization; the meaning you attach to the resulting song is yours. The honest split is simple: the music theory is exact and checked, the mapping from life to music is an artistic gesture.